The colonies are small, glistening and usually, the growth is irregular due to swarming. The Swarming of Proteus vulgaris is best observed in Nutrient Agar medium, covering the whole plate. PROTEUS VULGARIS CULTURE ON MACCONKEY AGAR MEDIUM Moreover, the swarming is inhibited in the MacConkey Agar medium due to the presence of Bile Salts. aureus), E.coli & Klebsiella species which are lactose fermentors and gives Pink colored colonies on MacConkey agar medium. vulgaris from other Bacteria present in the specimen, especially from Gram-positive bacteria ( S. In MacConkey Agar medium, the colonies of Proteus Vulgaris are pale or colorless due to lack of lactose fermentation which is of great importance in differentiating Pr. PROTEUS VULGARIS CULTURE ON BLOOD AGAR MEDIUM Usually, it produces non-hemolytic growth on Blood agar plate but some of the strains are β-Hemolytic. In Blood Agar medium, the Proteus Vulgaris exhibits the swarming and the growth occurs in the whole media plate. In Liquid culture media like Trypticase soy broth or Nutrient broth & Peptone Water, the growth of the bacterium occurs as a uniform turbidity in the broth medium with a powdery deposit and Ammoniacal smell which is further analyzed for the morphology (under the microscope), gram reaction, biochemical tests, and Proteus Vulgaris specific tests. MORPHOLOGY AND CULTURE CHARACTERISTICS OF KLEBSIELLA PNEUMONIAE (K. VULGARIS)Ĭheck out the Morphology & Culture Characteristics of Klebsiella pneumoniae CULTURE CHARACTERISTICS OF PROTEUS VULGARIS (PR. ⇒ The Eosin Methylene Blue Agar (EMB Agar) medium can also be used for the cultivation of Proteus Vulgaris in laboratory contains Eosin dye and Methylene Blue dye which inhibits the growth of many gram-positive bacteria, inhibits the growth of Shigella and Salmonella species and supports the growth of Proteus Vulgaris. The liquid medium ( Nutrient Broth medium, TSB medium, etc.).Eosin Methylene Blue Agar (EMB Agar) Medium.Xylose Lysine Dextrose (XLD) Agar medium.⇒ There are various culture media used for the cultivation of Proteus Vulgaris in the laboratory and most commonly the Nutrient Agar medium and MacConkey Agar medium is used, the other media are as follows – grow best in the presence of oxygen and it is also a Facultative anaerobic organism i.e. ⇒ Oxygen requirements – Proteus Vulgaris (Pr. MORPHOLOGY AND CULTURE CHARACTERISTICS OF SHIGELLA DYSENTERIAE (SH.
![proteus vulgaris gram stain proteus vulgaris gram stain](https://slideplayer.com/slide/5816437/19/images/13/Proteus+vulgaris+1000x+Gram+stain+2000x+flagellar.jpg)
Also, the pH requirement varies as per the strain of Proteus vulgaris.Ĭheck out the Morphology & Culture Characteristics of Shigella dysenteriae vulgaris can survive alkaline pH as well as acidic pH ranging from 4.0 – 11.0 and the maximum growth of the organism is observed at 6.0 i.e. ⇒ Optimum temperature – Proteus Vulgaris ranges from 10–43☌ but the optimum temperature for most of the strains is 37☌ and usually cultivated at this temperature in laboratories. Commonly the NAM & MacConkey Agar medium is used for the cultivation of Proteus Vulgaris in Laboratory. ⇒ Special requirements – Proteus Vulgaris have no complex nutritional requirements and readily grow in an ordinary media like Nutrient Agar medium (NAM). Gram Staining Reaction – Proteus Vulgaris is a Gram -ve (Negative) bacterium.ĬULTURE REQUIREMENTS OF PROTEUS VULGARIS (PR. Spores – The Proteus Vulgaris is a non–sporing bacterium.Ĭapsule – Capsules are not present in Proteus Vulgaris.
![proteus vulgaris gram stain proteus vulgaris gram stain](https://microbiologylearning.weebly.com/uploads/4/7/4/4/47442993/1817136_orig.jpg)
flagella are present all over the surface of the bacterium. Vulgaris is a flagellated bacterium with a Peritrichous flagella arrangement i.e.
![proteus vulgaris gram stain proteus vulgaris gram stain](https://i.pinimg.com/originals/a9/ac/04/a9ac042fe3bfee7966e3bb188ab58fbc.jpg)
Motility – Proteus Vulgaris is an actively motile bacterium and well known for the swarming growth on an ordinary medium like NAM.įlagella – Pr. MORPHOLOGY OF PROTEUS VULGARIS (MICROSCOPIC VIEW) vulgaris is arranged singly, in pairs, or in short chains and sometimes in clusters. Size – The size of Proteus Vulgaris is about 1–3 µm × 0.5 µm (micrometer).Īrrangement Of Cells – Pr. Shape – Proteus Vulgaris is a short, straight rod shape (bacillus) bacterium. That’s all about the Morphology & Culture Characteristics of Proteus vulgaris.CULTURE CHARACTERISTICS OF PROTEUS VULGARIS (PR.CULTURE REQUIREMENTS OF PROTEUS VULGARIS (PR.